Cervantes, Miriam P.Palomino Cano, Jesús ManuelAnzar, MuhammadMapletoft, Reuben J.Mastromonaco, Gabriela F.Adams, Gregg P.2023-04-122023-04-122017https://www.sciencedirect.com/science/article/pii/S0093691X1630509X?via%3Dihubhttp://146.190.124.33/handle/123456789/6562Experiments were conducted in wood bison to determine the effect of additional maturation time on embryo development of in vivo matured oocytes. In experiment 1, cumulus-oocyte complexes (COC) were collected 30 hours after hCG treatment in superstimulated wood bison, and expanded COC were fertilized immediately or after 4 hours of additional in vitro maturation. Embryo development was assessed on Days 3, 7, and 8 (Day 0 = day of fertilization). No difference in cleavage rate was detected (55.3% vs. 60.5%, P = 0.82), but the Day 8 blastocyst rate was higher after an additional 4 hours of in vitro maturation time (44.7 vs. 18.4%, P = 0.03). In experiment 2, COC were collected at either 30 hours or 34 hours after hCG treatment. Expanded COC from the 30 hours group were fertilized after 4 hours of in vitro maturation, whereas those from the 34 hours group were fertilized immediately. A higher cleavage rate (74.3 vs. 57.0%) and blastocyst rate (54.1 vs. 37.2%) were found in the 34 hours group versus the 30 hours group (P < 0.05). In conclusion, an additional short period of in vitro maturation, or an extended period of in vivo maturation are beneficial for in vitro embryo production in wood bison.application/pdfengBLASTOCYSTOOCYTEINVITRO FERTILIZATIONINVITROINVIVO MATURATIONinfo:eu-repo/semantics/article10.1016/j.theriogenology.2016.10.017