High Prevalence of blaCTX-Min Fecal Commensal Escherichia coli from healthy children
| dc.date.accessioned | 2023-04-05T12:46:19Z | |
| dc.date.available | 2023-04-05T12:46:19Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Background: Antibiotic-resistant Escherichia coli can colonize the intestinal tract of healthy children, causing concern when antibiotic resistance is related to the presence of transferable mechanisms, such as extended-spectrum β-lactamases (ESBLs). Materials and Methods: Fecal samples from 41 healthy children from two villages of rural Peru were cultured on ceftriaxone-disks. ESBL production was confirmed with double disk synergy. In all ESBL-produced isolates, antibiotic susceptibility to 12 antibacterial agents was established by disk diffusion, while clonal relationships were determined by repetitive extragenic palindromic-polymerase chain reaction (REP-PCR). Presence of ST131 was determined using PCR. Results: Ceftriaxone-resistant microorganisms were recovered from 39 samples belonging to 22 out of 41 children (53.7%). Of these, 80 ceftriaxone-resistant and two ceftriaxone-intermediate E. coli from inside ceftriaxone-halos were confirmed as ESBL-producers. All isolates were multidrug-resistant. In 79/80 (98.8%) ceftriaxone-resistant isolates, the presence of blaCTX-M was detected alone (58 isolates, or together with other β-lactamase (blaTEM, 17 isolates; blaOXA-1-like, 3 isolates; blaTEM + blaOXA-1-like, 1 isolate), while in one isolate no such ESBL was identified. The two ceftriaxone-intermediate isolates recovered from the same sample, carried a blaTEM and blaSHV respectively. Thirty-four different clones were identified, with 4 clones being recovered from different samples from the same child. Twelve clones were disseminated among different children, including 5 clones disseminated between both villages. Two clones, accounting for 3 isolates and both recovered from the same children, belonged to E. coli ST131. Conclusion: This study demonstrates high prevalence of ESBL-carriers among healthy children living in a rural area of Peru, stressing the need for continuous surveillance and search for public health control measures. © 2022 Korean Society for Antimicrobial Therapy. All rights reserved. | |
| dc.identifier.issn | 20932340 | |
| dc.identifier.uri | https://doi.org/10.3947/ic.2021.0102 | |
| dc.identifier.uri | http://146.190.124.33/handle/123456789/6054 | |
| dc.language.iso | eng | es_ES |
| dc.publisher | Korean Society of Infectious Diseases, Korean Society for Antimicrobial Therapy, Korean Society for AIDS | es_ES |
| dc.source | Revista Peruana de Biología; Vol. 28 Núm. 1 | |
| dc.title | High Prevalence of blaCTX-Min Fecal Commensal Escherichia coli from healthy children | es_ES |
| dc.type | info:eu-repo/semantics/article | es_ES |
| dc.type.version | info:eu-repo/semantics/publishedVersion |