In vivo and in vitro maturation of oocytes collected from superstimulated wood bison (Bison bison athabascae) during the anovulatory and ovulatory seasons

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dc.contributor.authorCervantes, Miriam P.es_ES
dc.contributor.authorPalomino Cano, Jesús Manueles_ES
dc.contributor.authorAnzar, Muhammades_ES
dc.contributor.authorMapletoft, Reuben J.es_ES
dc.contributor.authorAdams, Gregg P.es_ES
dc.date.accessioned2023-04-12T12:30:16Z
dc.date.available2023-04-12T12:30:16Z
dc.date.issued2016
dc.description.abstractExperiments were done to compare the in vivo and in vitro maturational characteristics of cumulus-oocyte complexes (COC) collected from live wood bison. In Experiment 1 (anovulatory season), follicular ablation was done to synchronize follicle wave emergence among bison on Day −1, and FSH was given on Days 0 and 2. Bison were then assigned to 5 groups (n = 5/group) in which COC were collected by transvaginal follicle aspiration on Day 4 and either fixed immediately with no maturation (control), matured in vitro for 24 or 30 h, or collected on Day 5 after in vivo maturation for 24 or 30 h (i.e., after hCG treatment). In Experiment 2 (ovulatory season), bison were treated as described for Experiment 1, but PGF2α (cloprostenol) was given to control the luteal phase on Days −9 and 3. In both experiments, cumulus cell expansion was more extensive following in vivo than in vitro maturation, and the percentage of fully expanded COC was highest in the in vivo 30 h groups. Nuclear maturation occurred more rapidly in vitro; 60–70% of oocytes were at the MII stage 24 h after in vitro maturation while only 25–27% of oocytes had reached the MII stage after 24 h of in vivo maturation. In conclusion, nuclear maturation occurred more rapidly during in vitro vs. in vivo maturation, but was associated with less cumulus expansion than in vivo maturation. In vivo oocyte maturation was more complete at 30 vs. 24 h after hCG treatment. Season had no effect on the maturational capacity of wood bison oocytes.es_ES
dc.formatapplication/pdfes_ES
dc.identifier.doi10.1016/j.anireprosci.2016.09.001
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0378432016304110?via%3Dihub
dc.identifier.urihttp://146.190.124.33/handle/123456789/6565
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.ispartofAnimal Reproduction Science; Vol. 173, pp. 87-96
dc.sourceAnimal Reproduction Sciencees_ES
dc.subjectHCGes_ES
dc.subjectIN VITROes_ES
dc.subjectIN VIVO NUCLEAR MATURATIONes_ES
dc.subjectOOCYTEes_ES
dc.subjectWOOD BISONes_ES
dc.titleIn vivo and in vitro maturation of oocytes collected from superstimulated wood bison (Bison bison athabascae) during the anovulatory and ovulatory seasonses_ES
dc.typeinfo:eu-repo/semantics/article
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