Determinación de algunas características biológicas de aislados primarios de células del corion de placenta de alpaca (Vicugna pacos) [Determination of some biological characteristics of primary isolates of chorion cells from the alpaca placenta (Vicugna pacos)]

dc.date.accessioned2023-04-05T12:45:53Z
dc.date.available2023-04-05T12:45:53Z
dc.date.issued2021
dc.description.abstractThe aim of this work was to determine the morphology and growth kinetics of an isolate of primary cells from passages 1-3 of the alpaca placental chorion (aPChC) as stem cell precursors. Five alpaca placentas were processed by mechanical enzymatic disintegration for primary isolation of nucleated cells, evaluating the shape, length, width, and kinetics of these cells, as well as the population of cells obtained per gram of placental chorion tissue. The results in cell isolation yielded 6x105 nucleated cells/g of chorion tissue. The morphometry evaluated from passage 0 to 3 showed longitudinal elongation ranging from 110.99 ± 27.16 µm to 298.24 ± 52.06 µm, respectively, while the measure of the cell width ranges from 30.97 ± 8.53 µm to 48.17 ± 11.27 µm. The growth kinetics expressed as cumulative population doubling level per passage number were 2.07, 1.65, 1.06 and 0.30. The morphology and growth kinetics characteristics of the first passages of the aPChC culture denote characteristics like those of stem cells of reported domestic species. This preliminary information is important for future characterization and expansion studies of aPChC for regenerative therapy purposes for South American camelids. © Los autores.
dc.identifier.issn16823419
dc.identifier.urihttp://www.scielo.org.pe/scielo.php?script=sci_arttext&pid=S1609-91172021000400015es_PE
dc.identifier.urihttps://cris.cientifica.edu.pe/en/publications/determinaci%C3%B3n-de-algunas-caracter%C3%ADsticas-biol%C3%B3gicas-de-aislados-pes_PE
dc.identifier.urihttps://siis.unmsm.edu.pe/en/publications/determinaci%C3%B3n-de-algunas-caracter%C3%ADsticas-biol%C3%B3gicas-de-aislados-p/fingerprints/es_PE
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=85116076171&partnerID=8YFLogxKes_PE
dc.language.isospaes_ES
dc.publisherUniversidad Nacional Mayor de San Marcoses_ES
dc.sourceInternational Orthodontics; Vol. 18 Núm. 4
dc.titleDeterminación de algunas características biológicas de aislados primarios de células del corion de placenta de alpaca (Vicugna pacos) [Determination of some biological characteristics of primary isolates of chorion cells from the alpaca placenta (Vicugna pacos)]es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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