Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa

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dc.contributor.authorEvangelista-Vargas, S.es_ES
dc.contributor.authorSantiani, A.es_ES
dc.date.accessioned6/22/2022 13:33
dc.date.accessioned2022-09-30T16:31:53Z
dc.date.available6/22/2022 13:33
dc.date.available2022-09-30T16:31:53Z
dc.date.issued2017
dc.description.abstractContents: The objective of this study was to detect changes in intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) production and lipid peroxidation during cryopreservation of alpaca spermatozoa. Twelve alpaca semen samples were conventionally cryopreserved. Intracellular superoxide anion and hydrogen peroxide were evaluated by fluorescence microscopy using dihydroethidium (DHE)/YO-PRO-1 and dichlorofluorescein diacetate (H2DCFDA)/propidium iodide (PI), respectively. Evaluations were performed during cooling curve at (1) 25°C, (2) 15°C, (3) 5°C/0 min, (4) 5°C/15 min, (5) 5°C/30 min and (6) after freezing/thawing. Evaluation of lipid peroxidation by measuring malondialdehyde (MDA) was performed at 25°C, 5°C/30 min and after thawing. Maximum percentages of total spermatozoa producing superoxide anion and hydrogen peroxide were found at 5°C/30 min (62.8 ± 6.3% and 30.5 ± 5.6%, respectively), and these results were higher (p < .05) than initial (25°C: 10.8 ± 3.8% and 6.8 ± 0.7%, respectively) and after thawing (29.8 ± 9.5% and 7.5 ± 1.8%, respectively) values. However, considering only viable spermatozoa, production of superoxide anion and hydrogen peroxide during overall stabilization at 5°C (>76% and >91%, respectively) and after thawing (74.9 ± 5.0% and 78.9 ± 2.2%, respectively) was higher (p < .05) than initial values at 25°C (38.7 ± 3.1% and 53.6 ± 2.0%, respectively). Lipid peroxidation at 25°C, 5°C/30 min, and post-thawing were 346.5 ± 99.8, 401.1 ± 64.8 and 527.7 ± 142.8 ng/ml MDA, respectively. These results showed that high percentage of viable alpaca spermatozoa produces intracellular reactive species oxygen (ROS) during the cryopreservation process of alpaca semen. © 2017 Blackwell Verlag GmbH.es_ES
dc.formatapplication/pdfes_ES
dc.identifier.doi10.1111/rda.12984es_ES
dc.identifier.urihttps://doi.org/10.1111/rda.12984
dc.language.isoenges_ES
dc.publisherBlackwell Publishing Ltdes_ES
dc.rightsinfo:eu-repo/semantics/closedAccesses_ES
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/es_ES
dc.sourceReproduction in Domestic Animalses_ES
dc.subjectAgricultural and Biological Scienceses_ES
dc.subjectBiochemistry, Genetics and Molecular Biologyes_ES
dc.subject.ocdehttp://purl.org/pe-repo/ocde/ford#4.01.00es_ES
dc.titleDetection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
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