Ovarian superstimulation and oocyte collection in wood bison (Bison bison athabascae) during the ovulatory season

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dc.contributor.authorPalomino Cano, Jesús Manueles_ES
dc.contributor.authorMcCorkell, Robert B.es_ES
dc.contributor.authorWoodbury, Murray R.es_ES
dc.contributor.authorCervantes, Miriam P.es_ES
dc.contributor.authorAdams, Gregg P.es_ES
dc.date.accessioned2023-04-12T12:30:16Z
dc.date.available2023-04-12T12:30:16Z
dc.date.issued2014
dc.description.abstractThe objective of the study was to establish an effective ovarian superstimulatory protocol and subsequently obtain oocytes from bison by transvaginal ultrasound-guided follicular aspiration. Two experiments involving 22 wood bison were done during the breeding season (September to December). In experiment 1, the bison were given a luteolytic dose of prostaglandin (Day 0) and underwent follicular ablation (Day 8) to induce ovarian synchrony. Synchronized bison were then assigned randomly to two groups (n = 11 per group) and given either 200 mg FSH diluted in saline sc, or 200 mg FSH diluted in a proprietary slow-release formulation (SRF) im on Days 9 and 11. Prostaglandin was given to both groups on Day 11 followed by 25 mg LH on Day 13. Oocytes were collected by transvaginal ultrasound-guided aspiration of follicles ≥5 mm on Day 14. In experiment 2, bison were synchronized as in experiment 1 and assigned randomly to one of two groups (n = 11 per group) and given either a single dose of 2500 IU eCG im on Day 9, or 200 mg FSH sc on Days 9 and 11. Prostaglandin was given to both groups on Day 11, and LH (25 mg) was given on Day 13. Oocyte collection was done as described in experiment 1. Cumulus-oocyte-complexes (COC) were classified according to morphologic characteristics. In experiment 1, more follicles ≥5 mm were detected on Day 14 in bison treated with FSH versus eCG (12.2 ± 1.73 vs. 5.8 ± 0.52; P < 0.05), and more COC were collected from FSH-treated animals (7.2 ± 1.41 vs. 3.4 ± 0.62; P < 0.05). In experiment 2, the FSH-saline and FSH-SRF groups had a similar number (mean value ± standard error of the mean) of follicles ≥5 mm on Day 14 (12.4 ± 1.49 vs. 13.8 ± 1.24, respectively) and a similar number of COC were collected (6.5 ± 1.13 vs. 6.3 ± 0.96, respectively). The proportion of COC collected per follicle aspirated and the percentage of compact, expanded, and denuded oocytes did not differ between groups in either experiment 1 or 2. In summary, a two-dose regimen of FSH diluted in saline and given sc or in a SRF and given im induced a similar ovarian response in wood bison, whereas a single dose of eCG resulted in a significantly lower ovarian response. Overall, COC were collected from 55% of follicles after transvaginal, ultrasound-guided needle aspiration in wood bison.es_ES
dc.formatapplication/pdfes_ES
dc.identifier.doi10.1016/j.theriogenology.2013.09.019
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0093691X13003841?via%3Dihub
dc.identifier.urihttp://146.190.124.33/handle/123456789/6568
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.ispartofTheriogenology; Vol. 81, N° 2, pp.250-256
dc.sourceTheriogenologyes_ES
dc.subjectECGes_ES
dc.subjectFSHes_ES
dc.subjectLHes_ES
dc.subjectOVARIAN SUPERSTIMULATIONes_ES
dc.subjectOOCYTE COLLECTIONes_ES
dc.subjectWOOD BISONes_ES
dc.titleOvarian superstimulation and oocyte collection in wood bison (Bison bison athabascae) during the ovulatory seasones_ES
dc.typeinfo:eu-repo/semantics/article
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