Antimicrobial efficacy of calcium and sodium hypochlorite at different concentrations on a biofilm of enterococcus faecalis and candida albicans: an in vitro comparative study
| dc.date.accessioned | 2023-04-05T12:45:41Z | |
| dc.date.available | 2023-04-05T12:45:41Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Aim: To compare the antimicrobial efficacy of sodium hypochlorite (NaClO at 2.5% and 5.25%) and calcium hypochlorite [Ca(ClO)2 at 2.5%] on a biofilm of Enterococcus faecalis ATCC 29212™ and Candida albicans ATCC 10231™. Materials and methods: We performed an experimental in vitro study. Strains of C. albicans and E. faecalis, which had previously been reactivated were used. Then the colonies to be used were standardized in a turbidity standard to guarantee a quantity of 108 (CFU/mL) using the McFarland scale (0.5). Subsequently, the biofilm formed in brain-heart infusion agar was seeded into 42 sterile disks previously embedded with the experimental substances. Both 2.5% NaClO and Ca(ClO)2 solutions were placed in each Petri dish. They were then incubated at 37°C for 24 hours and the inhibition halos were measured using the Kirby-Bauer technique. Results: The means between the halos corresponding to NaClO and Ca(ClO)2 at 2.5% were 13.38 ± 0.64 mm and 13.42 ± 0.62 mm, respectively. According to the Tukey test, no statistically significant differences were found between the hypochlorite groups evaluated (p = 0.989). Conclusion: Both Ca(ClO)2 and NaClO have a similar antimicrobial efficacy with biofilm based on E. faecalis and C. albicans, with no statistically significant differences between the two. Clinical significance: This study demonstrates the effectiveness of Ca(ClO)2 and NaClO as endodontic irrigators to combat the most frequent microorganisms of the root canal. © The Author(s). | |
| dc.identifier.issn | 15263711 | |
| dc.identifier.uri | https://doi.org/10.5005/jp-journals-10024-2771 | |
| dc.identifier.uri | https://pubmed.ncbi.nlm.nih.gov/32381824/ | es_PE |
| dc.language.iso | eng | es_ES |
| dc.publisher | Jaypee Brothers Medical Publishers (P) Ltd | es_ES |
| dc.source | Journal of Pure and Applied Microbiology; Vol. 14 Núm. 1 | |
| dc.title | Antimicrobial efficacy of calcium and sodium hypochlorite at different concentrations on a biofilm of enterococcus faecalis and candida albicans: an in vitro comparative study | es_ES |
| dc.type | info:eu-repo/semantics/article | es_ES |
| dc.type.version | info:eu-repo/semantics/publishedVersion |