In vitro embryo production in wood bison (Bison bison athabascae) using in vivo matured cumulus-oocyte complexes

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dc.contributor.authorCervantes, Miriam P.es_ES
dc.contributor.authorPalomino Cano, Jesús Manueles_ES
dc.contributor.authorAnzar, Muhammades_ES
dc.contributor.authorMapletoft, Reuben J.es_ES
dc.contributor.authorMastromonaco, Gabriela F.es_ES
dc.contributor.authorAdams, Gregg P.es_ES
dc.date.accessioned2023-04-12T12:30:16Z
dc.date.available2023-04-12T12:30:16Z
dc.date.issued2017
dc.description.abstractExperiments were conducted in wood bison to determine the effect of additional maturation time on embryo development of in vivo matured oocytes. In experiment 1, cumulus-oocyte complexes (COC) were collected 30 hours after hCG treatment in superstimulated wood bison, and expanded COC were fertilized immediately or after 4 hours of additional in vitro maturation. Embryo development was assessed on Days 3, 7, and 8 (Day 0 = day of fertilization). No difference in cleavage rate was detected (55.3% vs. 60.5%, P = 0.82), but the Day 8 blastocyst rate was higher after an additional 4 hours of in vitro maturation time (44.7 vs. 18.4%, P = 0.03). In experiment 2, COC were collected at either 30 hours or 34 hours after hCG treatment. Expanded COC from the 30 hours group were fertilized after 4 hours of in vitro maturation, whereas those from the 34 hours group were fertilized immediately. A higher cleavage rate (74.3 vs. 57.0%) and blastocyst rate (54.1 vs. 37.2%) were found in the 34 hours group versus the 30 hours group (P < 0.05). In conclusion, an additional short period of in vitro maturation, or an extended period of in vivo maturation are beneficial for in vitro embryo production in wood bison.es_ES
dc.formatapplication/pdfes_ES
dc.identifier.doi10.1016/j.theriogenology.2016.10.017
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0093691X1630509X?via%3Dihub
dc.identifier.urihttp://146.190.124.33/handle/123456789/6562
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.ispartofTheriogenology; Vol. 89, pp. 122-130
dc.sourceTheriogenologyes_ES
dc.subjectBLASTOCYSTes_ES
dc.subjectOOCYTEes_ES
dc.subjectINVITRO FERTILIZATIONes_ES
dc.subjectINVITROes_ES
dc.subjectINVIVO MATURATIONes_ES
dc.titleIn vitro embryo production in wood bison (Bison bison athabascae) using in vivo matured cumulus-oocyte complexeses_ES
dc.typeinfo:eu-repo/semantics/article
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