Efectos de isoniacida, vigabatrina, gabapentina y extracto pentánico de Lepidium meyenii L. (maca) sobre los niveles de GABA producidos por neuroblastoma B35
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Fecha
2014
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Universidad Católica de Santa María
Resumen
El ácido-γ-aminobutírico (GABA) es un neurotransmisor inhibitorio crucial en el sistema nervioso central. Se estudiaron cultivos celulares de neuroblastoma B35 para analizar la producción de GABA, utilizando cromatografía líquida de alta performance con detección electroquímica (HPLC-ED). Se observó que el neuroblastoma B35 sintetiza GABA, alcanzando niveles de 131.14±1.01 ng/mL a las 96 horas. Se evaluó el efecto de vigabatrina (VGT), gabapentina (GB), isoniacida (INH) y extracto pentánico de Lepidium meyenii L. (EM) sobre la producción de GABA. INH disminuyó los niveles de GABA, mientras que EM los incrementó. GB (1.0 mM) aumentó significativamente los niveles de GABA, atenuando la inhibición producida por INH. Se concluyó que EM (10 μg/mL) incrementa significativamente los niveles de GABA en células desarrolladas. Los tratamientos no afectaron la viabilidad celular.
Abstract Gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter crucial in the central nervous system. Neuroblastoma B35 cell cultures were studied to analyse GABA production, using high-performance liquid chromatography with electrochemical detection (HPLC-ED). It was observed that neuroblastoma B35 synthesises GABA, reaching levels of 131.14±1.01 ng/mL at 96 hours. The effects of vigabatrin (VGT), gabapentin (GB), isoniazid (INH), and pentane extract of Lepidium meyenii L. (EM) on GABA production were evaluated. INH decreased GABA levels, while EM increased them. GB (1.0 mM) significantly increased GABA levels, attenuating the inhibition caused by INH. It was concluded that EM (10 μg/mL) significantly increases GABA levels in developed cells. The treatments did not affect cell viability.
Abstract Gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter crucial in the central nervous system. Neuroblastoma B35 cell cultures were studied to analyse GABA production, using high-performance liquid chromatography with electrochemical detection (HPLC-ED). It was observed that neuroblastoma B35 synthesises GABA, reaching levels of 131.14±1.01 ng/mL at 96 hours. The effects of vigabatrin (VGT), gabapentin (GB), isoniazid (INH), and pentane extract of Lepidium meyenii L. (EM) on GABA production were evaluated. INH decreased GABA levels, while EM increased them. GB (1.0 mM) significantly increased GABA levels, attenuating the inhibition caused by INH. It was concluded that EM (10 μg/mL) significantly increases GABA levels in developed cells. The treatments did not affect cell viability.
Descripción
niversidad Católica de Santa María, Facultad de Ciencias Farmacéuticas, Bioquímicas y Biotecnológicas, Programa Profesional de Farmacia y Bioquímica. Tesis para optar el título profesional de Químico Farmacéutico.
Asesor PhD. Jaime Cárdenas García
Asesor PhD. Jaime Cárdenas García
Palabras clave
Neuroblastoma, Lepidium Meyenii (Maca)