Evaluación de la actividad de extractos y aceites vegetales de plantas de la región contra Proteus mirabilis uropatogénico
Archivos
Fecha
2013
Autores
Título de la revista
ISSN de la revista
Título del volumen
Editor
Universidad de la República Uruguay
Resumen
Este estudio se centró en el cribado de extractos vegetales y aceites esenciales para evaluar su efecto en el crecimiento o la virulencia de Proteus mirabilis, causante de infecciones del tracto urinario. Se evaluaron 28 extractos vegetales, incluidos 5 aceites (2 extractos oleosos y 3 aceites esenciales). Los aceites esenciales mostraron las concentraciones inhibitorias mínimas más bajas, mientras que los extractos con mejor efecto bacteriostático fueron Acalypha communis, Fluorensia oolepis, Vernonia rubricaulis, Achyrocline saturoides, Lithraea molleoides y Melia azedarach (extracto etanólico). Se realizaron ensayos de hemaglutinación, movilidad swarming y formación de biofilms para evaluar la acción de los extractos contra factores de virulencia. El extracto de Lithraea molleoides fue seleccionado para estudios cromatográficos adicionales para identificar los compuestos responsables de su acción.
Abstract This study focused on screening plant extracts and essential oils for their effects on the growth or virulence of Proteus mirabilis, a causative agent of urinary tract infections. Twenty-eight plant extracts were evaluated, including five oils (two oily extracts and three essential oils). Essential oils showed the lowest minimum inhibitory concentrations, while the extracts with the best bacteriostatic effect were Acalypha communis, Fluorensia oolepis, Vernonia rubricaulis, Achyrocline saturoides, Lithraea molleoides, and Melia azedarach (ethanolic extract). Hemagglutination, swarming motility, and biofilm formation assays were conducted to evaluate the extracts' action against virulence factors. The extract of Lithraea molleoides was selected for further chromatographic studies to identify the compounds responsible for its action.
Abstract This study focused on screening plant extracts and essential oils for their effects on the growth or virulence of Proteus mirabilis, a causative agent of urinary tract infections. Twenty-eight plant extracts were evaluated, including five oils (two oily extracts and three essential oils). Essential oils showed the lowest minimum inhibitory concentrations, while the extracts with the best bacteriostatic effect were Acalypha communis, Fluorensia oolepis, Vernonia rubricaulis, Achyrocline saturoides, Lithraea molleoides, and Melia azedarach (ethanolic extract). Hemagglutination, swarming motility, and biofilm formation assays were conducted to evaluate the extracts' action against virulence factors. The extract of Lithraea molleoides was selected for further chromatographic studies to identify the compounds responsible for its action.
Descripción
Universidad de la República Uruguay, Facultad de Ciencias. Tesina para optar el grado de Licenciatura de Bioquímica.
Asesor M.Sc. Vanessa Sosa
Asesor M.Sc. Vanessa Sosa
Palabras clave
Microbiología, Proteus Mirabilis, Enfermedades Bacterianas, Enfermedades Infecciosas, Bacterias, Plantas Medicinales, Uruguay