Examinando por Autor "Pradel, P."

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    ÍtemAcceso Abierto
    Draft genome sequence of a copper-resistant marine bacterium, Pantoea agglomerans strain LMAE-2, a bacterial strain with potential use in bioremediation
    (American Society for Microbiology, 2016) Corsini, G.; Valdés, N.; Pradel, P.; Tello, M.; Cottet, L.; Muiño, L.; Karahanian, E.; Castillo, A.; Gonzalez, A.R.
    Pantoea agglomerans LMAE-2 was isolated from seabed sediment moderately contaminated with Cu2+. Here, we report its draft genome sequence, which has a size of 4.98 Mb. The presence of cop genes related with copper homeostasis in its genome may explain the resistance and strengthen its potential for use as bioremediation agent. © 2016 Corsini et al.
  • Miniatura
    ÍtemAcceso Abierto
    Isolation and molecular characterization of Thraustochytrium strain isolated from Antarctic Peninsula and its biotechnological potential in the production of fatty acids
    (Elsevier Editora Ltda, 2017) Caamaño, E.; Loperena, L.; Hinzpeter, I.; Pradel, P.; Gordillo F.; Corsini, G.; Tello, M.; Lavín, P.; González, A.R.
    Thraustochytrids are unicellular protists belonging to the Labyrinthulomycetes class, which are characterized by the presence of a high lipid content that could replace conventional fatty acids. They show a wide geographic distribution, however their diversity in the Antarctic Region is rather scarce. The analysis based on the complete sequence of 18S rRNA gene showed that strain 34-2 belongs to the species Thraustochytrium kinnei, with 99% identity. The total lipid profile shows a wide range of saturated fatty acids with abundance of palmitic acid (16:0), showing a range of 16.1–19.7%. On the other hand, long-chain polyunsaturated fatty acids, mainly docosahexaenoic acid and eicosapentaenoic acid are present in a range of 24–48% and 6.1–9.3%, respectively. All factors analyzed in cells (biomass, carbon consumption and lipid content) changed with variations of culture temperature (10 °C and 25 °C). The growth in glucose at a temperature of 10 °C presented the most favorable conditions to produce omega-3fatty acid. This research provides the identification and characterization of a Thraustochytrids strain, with a total lipid content that presents potential applications in the production of nutritional supplements and as well biofuels. © 2017 Sociedade Brasileira de Microbiologia