Examinando por Autor "Lewis, C.M. Jr."

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  • Miniatura
    ÍtemAcceso Abierto
    Ezakiella peruensis gen. nov., sp. nov. isolated from human fecal sample from a coastal traditional community in Peru
    (Academic Press, 2015) Patel, N.B.; Tito, R.Y.; Obregón-Tito, A.J.; O'Neal, L.; Trujillo-Villaroel, O.; Marin-Reyes, L.; Troncoso-Corzo, L.; Guija-Poma, E.; Hamada, M.; Uchino, Y.; Lewis, C.M. Jr.; Lawson, P.A.
    A novel Gram-stain positive, non-motile, non-sporeforming coccus-shaped, obligately anaerobic bacterium was isolated from a fecal sample of an individual residing in a traditional Peruvian community. The organism was characterized using biochemical, chemotaxonomic and phylogenetic methods. Comparative 16S rRNA gene sequence analyses and phenotypic characteristics demonstrated that the organism was biochemically and phenotypically related, but distinct, from a group of organisms referred to as the Gram-stain positive anaerobic cocci (GPAC). The major cellular fatty acids of the novel isolate were determined to be C16:0 (18.3%), C18:1ω9c (39.8%), C18:2ω6,9c/C18:0 ANTE (13.2%). Fermentation end products from PYG are acetate and formate. Cell-wall peptidoglycan was found to be A4α (L-Lys-L-Ala-L-Glu) and the G+C content was determined to be 38.4mol%. Based on the phenotypic, chemotaxonomic, and phylogenetic results, Ezakiella peruensis gen. nov., sp. nov., is now proposed. The type strain is M6.X2T (DSM 27367T=NBRC 109957T=CCUG 64571T). © 2014.
  • Miniatura
    ÍtemAcceso Abierto
    Peptoniphilus catoniae sp. Nov., isolated from a human faecal sample from a traditional peruvian coastal community
    (Microbiology Society, 2016) Patel, N.B.; Tito, R.Y.; Obregón-Tito, A.J.; O’Neal, L.; Trujillo-Villaroel, O.; Marin-Reyes, L.; Troncoso-Corzo, L.; Guija-Poma, E.; Lewis, C.M. Jr.; Lawson, P.A.
    A novel Gram-stain-positive, coccus-shaped, obligately anaerobic bacterium was isolated from a faecal sample obtained from an individual in a traditional community located off the southern coast of Peru. Comparative 16S rRNA gene sequence analysis showed the novel bacterium belonged to the genus Peptoniphilus but showed no particular relationship with any species, demonstrating less than 91% 16S rRNA gene sequence similarity with all members of the genus. The major cellular fatty acids of the novel isolate were determined to be C10: 0, C14: 0, C16: 0, C18: 1ω9c and C18: 2ω6,9c/anteiso-C18: 0. The DNA G+C content was 34.4 mol%. End-products of metabolism from peptone-yeast-glucose broth (PYG) were determined to be acetate and butyrate. Based on the phenotypic, chemotaxonomic and phylogenetic results, the organism represents a novel species of the genus Peptoniphilus, for which the name Peptoniphilus catoniae sp. nov. is proposed. The type strain is M6.X2DT (=DSM 29874T=CCUG 66798T). © 2016 IUMS.